For improved biosynthesis of penicillin amidase by E. coli, NCIM 2400, the participation of different carboxylic acids and polyols has been studied in association with the effect of phenylacetic acid. A modified defined medium has been devised for this purpose.
Role ofrelAmutation in the survival of amino acid-starvedEscherichia coli
✍ Scribed by Michael Hecker; Andreas Schroeter; Kersten Träder; Friedrich Mach
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 585 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Amino acid-starved cells of Escherichia coli relA +, which contain a targe number of glycogen part,ties, are able to survive in phosphate buffer for a longer time period than their relaxed counterparts. With regard to NH f starvation differences in the survival of both strains were not found.
H + N 4 starved cells of E. coli relA are able to synthesize glycogen but amino acid-starved cells of the reIA strain are not. We suggest that the synthesis of glycogen triggered by guanosine tetraphosphate during amino acid starvation is responsible for the prolonged viability of the E. coli relA + strain.
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