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Role of xylose transporters in xylitol production from engineered Escherichia coli

✍ Scribed by Reza Khankal; Jonathan W. Chin; Patrick C. Cirino

Book ID
118472606
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
474 KB
Volume
134
Category
Article
ISSN
0168-1656

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The range of value-added chemicals produced by Escherichia coli from simple sugars has been expanded to include xylitol. This was accomplished by screening the in vivo activity of a number of heterologous xylitol-producing enzymes. Xylose reductases from Candida boidinii (CbXR), Candida tenuis (CtXR

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## Abstract __Escherichia coli__ strain PC09 (Ξ”__xylB__, cAMP‐independent CRP (__crp__\*) mutant) expressing an NADPH‐dependent xylose reductase from __Candida boidinii__ (CbXR) was previously reported to produce xylitol from xylose while metabolizing glucose [Cirino et al. (2006) Biotechnol Bioeng

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## Abstract __Escherichia coli__ engineered to uptake xylose while metabolizing glucose was previously shown to produce high levels of xylitol from a mixture of glucose and xylose when expressing NADPH‐dependent xylose reductase from __Candida boidinii__ (CbXR) (Cirino et al., __Biotechnol Bioeng__