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Role of Tryptophan in the Active Site of Plant Esterase: Chemical Modification and Fluorometric Studies

✍ Scribed by Limin Yang, Danqun Huo, Kun He, Suyi Zhang…


Book ID
120897081
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
608 KB
Volume
170
Category
Article
ISSN
0273-2289

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✍ Masatake Ohnishi; Yuki Nakamura; Masako Murata-Nakai; Keitaro Hiromi 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 541 KB

At pH 4.5 (the optimum), stopped-flow studies of the chemical modification with N-bromosuccinimide (NBS) of the tryptophan residues of glucoamylase from Rhizopus niveus clearly show reaction curves having two phises, fast and slow, in which two separate types of Trp residues are respectively involve