✦ LIBER ✦

Role of the H19 gene in Syrian hamster embryo cell tumorigenicity

✍ Scribed by Robert J. Isfort; David B. Cody; Gary A. Kerckaert; Benjamin Tycko; Robert A. LeBoeuf

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 132 KB
Volume: 20
Category: Article
ISSN: 0899-1987
DOI: 10.1002/(sici)1098-2744(199710)20:2<189::aid-mc5>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

Carcinogen-induced transformation in Syrian hamster embryo (SHE) cells is a multistage process characterized by specific genetic alterations at each stage in the transformation process. Loss of H19 gene expression is one of the earliest events observed, occurring in approximately 75% of the morphologically transformed cells and the subsequently derived tumorigenic cells. To investigate the effect the loss of H19 expression has on SHE cell tumorigenicity, H19 expression was reestablished in a tumorigenic SHE cell lineage that lacked H19 expression. H19 reexpression had little effect on cellular growth in vitro but did retard tumor growth in nude mice. Analysis of the tumors that did develop from cells containing the H19 gene indicated that loss of exogenous H19 gene expression was probably due to changes in DNA methylation. These results demonstrate that alterations in H19 gene expression play an important role in SHE cell tumorigenicity.

📜 SIMILAR VOLUMES

Clustering of discrete cell properties e

Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro

✍ G. I. Deichman; T. E. Kluchareva; V. A. Matveeva; N. E. Kushlinsky; L. S. Bassal 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 French ⚖ 431 KB

The expression of two discrete cell properties related to the host natural effector mechanisms, i.e., resistance to damage by H2O2, a cytotoxic product of activated macrophages, and the ability to secrete PGE, which inhibits NK-cell cytotoxicity, has been examined in parental Syrian hamster embryo c

Cell-transforming activity and genotoxic

Cell-transforming activity and genotoxicity of phenolphthalein in cultured Syrian hamster embryo cells

✍ Takeki Tsutsui; Yukiko Tamura; Eiichi Yagi; Koko Hasegawa; Yuriko Tanaka; Akira 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 83 KB

Phenolphthalein is a cathartic agent widely used in nonprescription laxatives. For the simultaneous assessment of in vitro carcinogenicity and mutagenicity of phenolphthalein, the ability of this chemical to induce cell transformation and genetic effects was examined using the Syrian hamster embryo

Clustering of discrete cell properties e

Clustering of discrete cell properties essential for tumorigenicity and metastasis. II. Studies of syrian hamster embryo fibroblasts transformed by rous sarcoma virus

✍ G. I. Deichman; H. A. Kashleva; T. E. Kluchareva; V. A. Matveeva 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 French ⚖ 400 KB

Tumorigenic and metastasizing activities (TGA; MA) and susceptibility, or resistance to H,O, and PGE-releasing activity (HzO: + PGE'+ phenotype) have been examined in 6 Syrian hamster embryo cell strains transformed in vitro with Rous sarcoma viruses (Schmidt-Ruppin and Prague strains). Early observ

Regulation of the aminolevulinate syntha

Regulation of the aminolevulinate synthase gene in the Syrian hamster Harderian gland: Changes during development and circadian rhythm and role of some hormones

✍ Rodríguez, Carmen; Kotler, Monica; Antolín, Isaac; Sainz, Rosa María; Menéndez-P 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 727 KB

The Syrian hamster Harderian gland has been advocated as a model to study the porphyrin biosynthetic pathway, since it shows by far the highest porphyrin concentration known to date. Another particular characteristic is the sexual dimorphism at both the morphological and the biochemical levels. We f

Mating-induced expression of c-fos in th

Mating-induced expression of c-fos in the male Syrian hamster brain: Role of experience, pheromones, and ejaculations

✍ Kollack-Walker, Sara ;Newman, Sarah W. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 898 KB

This study was designed to investigate the effects of pheromonal cues and specific behaviors within the male copulatory sequence on c-fos expression in the medial nucleus of the amygdala (Me), the bed nucleus of the stria terminalis (BNST), and the medial preoptic area (MPOA) of the Syrian hamster b

Involvement of genotoxic effects in the

Involvement of genotoxic effects in the initiation of estrogen-induced cellular transformation: Studies using Syrian hamster embryo cells treated with 17β-estradiol and eight of its metabolites

✍ Takeki Tsutsui; Yukiko Tamura; Eiichi Yagi; J. Carl Barrett 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 114 KB 👁 2 views

To examine a direct involvement of genotoxic effects of estrogens in the initiation of hormonal carcinogenesis, the abilities of 17beta-estradiol (E2) and 8 of its metabolites to induce cellular transformation and genetic effects were studied using the Syrian hamster embryo (SHE) cell model. Treatme