Role of the charge in continuous beds in the chiral separation of hydroxy acids by ligand-exchange capillary electrochromatography

✍ Scribed by Oliver Lecnik; Gerald Gübitz; Martin G. Schmid

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 79 KB
• Volume: 24
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200305525

✦ Synopsis

Abstract

This paper deals with the chiral separation of hydroxy acids using diallyl‐dimethylammonium chloride as a positive charge‐providing agent in the continuous bed. The chiral continuous bed was prepared by in situ copolymerization of monomers, including an L‐4‐hydroxyproline derivative as a chiral selector. This phase was applied to the chiral separation of hydroxy monocarboxylic acids and hydroxy dicarboxylic acids, respectively. The influence of both the selector concentration and the charge‐providing agent on retention and separation was investigated.