Role of protein phosphatases inhibitors in selenium-induced apoptosis in human hepatoma HepG2 cells
โ Scribed by H.H. Aydin; G. Saydam; H.A. Celik; S.B. Omay; Y.B. Batur
- Book ID
- 117372296
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 160 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0168-8278
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The mechanisms involved in the anti-carcinogenic activity of selenium remain to be elucidated. In the present study, we examined sodium selenite-induced oxidative stress and apoptosis in a human hepatoma cell line (HepG 2 ). Sodium selenite (10 M) exerted clear cytotoxic effect, as shown by the sign
S-adenosylmethionine (SAMe) and its metabolite 5-methylthioadenosine (MTA) are proapoptotic in HepG2 cells. In microarray studies, we found SAMe treatment induced Bcl-x expression. Bcl-x is alternatively spliced to produce two distinct mRNAs and proteins, Bcl-x L and Bcl-x S . Bcl-x L is antiapoptot