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Role of protein kinase C in transforming growth factor-β1 induction of carcinoembryonic antigen in human colon carcinoma cells

✍ Scribed by Subhas Chakrabarty

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
699 KB
Volume
152
Category
Article
ISSN
0021-9541

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✦ Synopsis

Transforming growth factor-pl (TGF-P1 ) regulates the expression of the carcinoembryonic antigen (CEA) gene family in the human colon carcinotna cell line Moscr. The mechanisms through which it acts, however, are unknown. In this communication, sevrral lines of evidence are presented to show that the induction of CEA expression and secretion (collectively called CEA response3) by TGF-p1 is associated with protein kinase C (PKC) pathway of signal transduction.

Trealnient of intact cells with the PKC-specific inhibitor calphostin C downmodulated cellular PKC phosphotransferase activity and blocked thc induction of the CEA responses by TGF-PI . Depletion of PKC by treatment of intact cells with phorbol ester also blocked the action of TGF-PI. The induction of the CEA responses by TGF-PI was also blocked by the protein kinase inhibitor 1 -(isoquin-

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