𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Role of mitogen-activated protein kinases in phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells

✍ Scribed by Dong Xiao; Sunga Choi; Yong J. Lee; Shivendra V. Singh

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
455 KB
Volume
43
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The present study was undertaken to examine the role of mitogen‐activated protein kinases (MAPKs) in apoptosis induction by phenethyl isothiocyanate (PEITC), a cruciferous vegetable‐derived cancer chemopreventive agent, with DU145 and LNCaP human prostate cancer cells as a model. The MAPK family of serine/threonine kinases, including extracellular signal‐regulated kinase1/2 (ERK1/2), c‐jun N‐terminal kinase1/2/3 (JNK1/2/3), and p38 MAPK play an important role in cell proliferation and apoptosis in response to different stimuli. Exposure of DU145 and LNCaP cells to growth suppressive concentrations of PEITC resulted in activation of ERK1/2 and JNKs, but not p38 MAPK, in both cell lines. In DU145 cells, the apoptosis induction by PEITC was statistically significantly attenuated by pharmacological inhibition of JNKs with SP600125. Adenovirus‐mediated overexpression of Flag‐tagged JNK binding domain (JBD) of JNK‐interacting protein‐1 (JIP‐1), an inhibitor of JNK, also inhibited PEITC‐induced apoptosis in DU145 cells. On the other hand, inhibition of ERK1/2 activation with MEK1 inhibitor PD98059 failed to offer protection against PEITC‐induced apoptosis in DU145 cells. In LNCaP cells, the PEITC‐induced cell death was not affected by either pretreatment with PD98059 or SP600125 or overexpression of JBD of JIP‐1. These results indicate that involvement of MAPKs in apoptosis induction by PEITC in human prostate cancer cells is cell line‐specific. © 2005 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Mitogen-activated protein kinases modula
Mitogen-activated protein kinases modulate H2O2-induced apoptosis in primary rat alveolar epithelial cells
✍ Helotonio Carvalho; Pablo Evelson; Samuel Sigaud; Beatriz González-Flecha 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 268 KB

Increasing evidence suggests a role for apoptosis in the maintenance of the alveolar epithelium under normal and pathological conditions. However, the signaling pathways modulating alveolar type II (ATII) cell apoptosis remain poorly defined. Here we investigated the role of MAPKs as modulators of o

Modulation of phosphatidylinositol-3-kin
Modulation of phosphatidylinositol-3-kinase/protein kinase B- and mitogen-activated protein kinase-pathways by tea polyphenols in human prostate cancer cells
✍ Imtiaz Ahmad Siddiqui; Vaqar Mustafa Adhami; Farrukh Afaq; Nihal Ahmad; Hasan Mu 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 471 KB

## Abstract We have earlier shown that oral infusion of a polyphenolic fraction isolated from green tea, at a human achievable dose (equivalent to six cups of green tea per day), significantly inhibits prostate cancer (PCA) development and metastasis in __tr__ansgenic __a__denocarcinoma of __m__ous

Naturally occurring asteriscunolide A in
Naturally occurring asteriscunolide A induces apoptosis and activation of mitogen-activated protein kinase pathway in human tumor cell lines
✍ Gledy Negrín; José L. Eiroa; Manuel Morales; Jorge Triana; José Quintana; Franci 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 384 KB 👁 1 views

Sesquiterpene lactones have attracted much attention because they display a wide range of biological activities, including antitumor properties. Here, we show the effects of the naturally occurring sesquiterpene lactone asteriscunolide A (AS) on viability of human melanoma, leukemia and cells that o

Contributions of mitogen-activated prote
Contributions of mitogen-activated protein kinase and nuclear factor kappa B to N-(4-hydroxyphenyl)retinamide–induced apoptosis in prostate cancer cells
✍ Keiji Shimada; Mitsutoshi Nakamura; Eiwa Ishida; Munehiro Kishi; Shin Yonehara; 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 419 KB

## Abstract The synthetic retinoid __N__‐(4‐hydroxyphenyl)retinamide (4‐HPR) has been shown to induce apoptosis in various types of tumors, including prostate cancer. We sought to examine the key mechanisms affecting the resistance to 4‐HPR–induced apoptosis in three human prostate cancer cell line