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Role of membrane potential in the regulation of lectin-induced calcium uptake

✍ Scribed by Erwin W. Gelfand; Roy K. Cheung; Sergio Grinstein

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
754 KB
Volume
121
Category
Article
ISSN
0021-9541

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✦ Synopsis

Human peripheral blood mononuclear cells were obtained following Ficoll-Hypaque gradient centrifugation (Cheung et al., 1982a). T lymphocytes were obtained following E-rosette enrichment as described (Cheung et al., 1982a). Greater than 95% of the cells obtained in this way were identified as T lymphocytes.

Media

Normal phosphate-buffered saline (PBS) was prepared as described (Cheung et al., 198213). Where indicated, the NaCl was isoosmotically substituted by KCl, RbCl, CsCI,

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