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Role of lysine-67 in the active site of class C β-lactamase from Citrobacter freundii GN346

✍ Scribed by Kikuo TSUKAMOTO; Katsumi TACHIBANA; Noriko YAMAZAKI; Yuko ISHII; Kumiko UJHE; Naoko NISHIDA; Tetsuo SAWAI


Book ID
115127713
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
924 KB
Volume
188
Category
Article
ISSN
1432-1327

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Re-examining the role of Lys67 in class
✍ Yu Chen; Andrea McReynolds; Brian K. Shoichet 📂 Article 📅 2009 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 742 KB

## Abstract Lys67 is essential for the hydrolysis reaction mediated by class C β‐lactamases. Its exact catalytic role lies at the center of several different proposed reaction mechanisms, particularly for the deacylation step, and has been intensely debated. Whereas a conjugate base hypothesis post