𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Role of leukemia inhibitory factor in the regulation of the proliferation and differentiation of neonatal mouse epidermal melanocytes in culture

✍ Scribed by Tomohisa Hirobe

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
271 KB
Volume
192
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Mouse epidermal melanoblasts/melanocytes preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in a serum‐free melanoblast/melanocyte‐proliferation medium supplemented with dibutyryl adenosine 3′:5′‐cyclic monophosphate (DBcAMP) and/or basic fibroblast growth factor (bFGF). Leukemia inhibitory factor (LIF) supplemented to the medium from initiation of primary culture increased the proliferation of melanoblasts or melanocytes as well as the differentiation of melanocytes. Pure cultured primary melanoblasts or melanocytes were further cultured with the medium supplemented with LIF from 14 days (keratinocyte depletion). LIF stimulated the proliferation of melanoblasts or melanocytes as well as the differentiation of melanocytes in the absence of keratinocytes. Moreover, anti‐LIF antibody supplemented to the medium from initiation of primary culture inhibited the proliferation of melanoblasts or melanocytes as well as the differentiation of melanocytes. These results suggest that LIF is one of the keratinocyte‐derived factors involved in regulating the proliferation and differentiation of neonatal mouse epidermal melanocytes in culture in cooperation with cAMP elevator and bFGF. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Stimulation of the proliferation and dif
Stimulation of the proliferation and differentiation of mouse pink-eyed dilution epidermal melanocytes by excess tyrosine in serum-free primary culture
✍ Tomohisa Hirobe; Kazumasa Wakamatsu; Shosuke Ito; Hiroyuki Abe; Yoko Kawa; Masak 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 307 KB

## Abstract The epidermal cell suspensions of the neonatal dorsal skin derived from wild type mouse at the pink‐eyed dilution (__p__) locus (black, C57BL/10JHir‐__P/P__) and their congenic mutant mouse (pink‐eyed dilution, C57BL/10JHir‐__p/p__) were cultured with a serum‐free melanocyte growth medi

The role of leukemia inhibitory factor i
The role of leukemia inhibitory factor in skeletal muscle regeneration
✍ John B. Kurek; John J. Bower; Margarita Romanella; Frank Koentgen; Mark Murphy; 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 390 KB 👁 2 views

Although a number of cytokines have been implicated in tissue regeneration, it is unknown which ones actually function in vivo. Here, we use mice with a targeted mutation in the leukemia inhibitory factor (LIF) gene to examine the role of LIF in muscle regeneration. Using a muscle crush model, we sh

ACTH4-12 is the minimal message sequence
ACTH4-12 is the minimal message sequence required to induce the differentiation of mouse epidermal melanocytes in serum-free primary culture
✍ Hirobe, Tomohisa; Abe, Hiroyuki 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 466 KB 👁 1 views

It is well known that α-melanocyte stimulating hormone (MSH) induces the differentiation of mouse epidermal melanocytes in vivo and in vitro. Although adrenocorticotropic hormone (ACTH) possesses the same amino acid sequence as MSH does, it is not clear whether the peptide and its fragments induce t

Isoform-specific role of transforming gr
Isoform-specific role of transforming growth factor-β2 in the regulation of proliferation and differentiation of murine adrenal chromaffin cells in vivo
✍ Belal Rahhal; Nicole Dünker; Stephanie Combs; Kerstin Krieglstein 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB 👁 2 views

## Abstract Chromaffin cells, the neuroendocrine cells of the adrenal medulla, play an important role in molecular, cellular, and developmental neurobiology. Unlike the closely related sympathetic neurons, chromaffin cells are able to proliferate throughout their whole life span. Proliferation of c

Leukemia inhibitory factor regulates the
Leukemia inhibitory factor regulates the timing of oligodendrocyte development and myelination in the postnatal optic nerve
✍ Tomoko Ishibashi; Philip R. Lee; Hiroko Baba; R. Douglas Fields 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB

## Abstract Leukemia inhibitory factor (LIF) promotes the survival of oligodendrocytes both in vitro and in an animal model of multiple sclerosis, but the possible role of LIF signaling in myelination during normal development has not been investigated. We find that LIF^−/−^ mice have a pronounced

Regulation of cyclooxygenase gene expres
Regulation of cyclooxygenase gene expression in rat endometrial stromal cells: The role of epidermal growth factor
✍ Bany, Brent M. ;Kennedy, Thomas G. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 183 KB 👁 3 views

Epidermal growth factor stimulates prostaglandin production and cyclooxygenase activity in endometrial stromal cells isolated from the uteri of ovariectomized rats sensitized for the decidual reaction. The present study examined the effect of epidermal growth factor on cyclooxygenase-1 and cyclooxyg