Role of laminin in axonal extension from olfactory receptor cells

✍ Scribed by Kafitz, Karl W. ;Greer, Charles A.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 337 KB
• Volume: 32
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199703)32:3<298::aid-neu4>3.0.co;2-2

✦ Synopsis

The role of laminin, an extracellulonger than on the poly-L-lysine control substrates. In lar matrix molecule believed to be involved in axon addition, the direction of the neurite outgrowth was extension, was explored in the outgrowth of olfactory receptor cells and therefore in the maintenance of orinfluenced by laminin. Fifty-six percent of all receptor ganization in the olfactory pathway. First, immunocycells located in a defined area surrounding a laminin tochemistry was used to examine laminin expression zone extended neurites onto laminin. In contrast, only in the olfactory nerve and bulb during development.

7% of all receptor cells located in the corresponding Laminin immunoreactivity was high in the olfactory laminin zone extended a neurite onto poly-L-lysine. In nerve and glomerular layers. Although it declined in summary, these data suggest that laminin provides a intensity, laminin expression continued in the nerve favorable substrate for the extension of the primary and in single glomeruli of adults. Second, the influence neurite from olfactory receptor cells and the direction of laminin on neurite outgrowth was examined in vitro of their extension. Therefore, laminin may be a factor using olfactory receptor cells harvested from E14 rat underlying continuous olfactory receptor cell axon embryos. We developed an in vitro assay to quantify outgrowth and its pathfinding in the olfactory system. the substrate preference of outgrowing neurites. Cells