Concentration-dependent effects of intracellular GTP on activation of the muscarinic K + channel were examined in inside-out patches of cardiac atrial myocytes. The pipette solution contained 0.i ~I ACh. GTP (0.01-30 ~M) and 0.5 mM MgCI 2 were applied to the inside side of the patch membrane. K + ch
Role of intracellular Mg2+in the activation of muscarinic K+channel in cardiac atrial cell membrane
β Scribed by Yoshihisa Kurachi; Toshiaki Nakajima; Tsuneaki Sugimoto
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 300 KB
- Volume
- 407
- Category
- Article
- ISSN
- 0031-6768
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The (Ca2+ + Mg2+) ATPase which serves as a Ca2+ pump in the kidney basolateral membranes is essential to the maintenance of an intracellular Ca2+ concentration optimal for kidney function. Since atrial natriuretic peptide (ANP) is known to participate in the Ca2+ homeostasis mechanism, altered level
While opening of cardiac muscarinic K+ channels is mediated by a guanine nucleotide-binding protein, GK, and normally requires both ACh and cytosolic GTP, we demonstrate that in the absence of agonist and G-nucleotides guinea-pig atrial muscarinic K+ channels are activated by cytosolic ATP (K50 = 22
M2-cholinergic receptor activation by acetylcholine (ACh) is known to cause a negative inotropic and chronotropic action in atrial tissues. This effect is still controversial in ventricular tissues. The ACh-sensitive muscarinic K+ channel (IK(ACh)) activity was characterized in isolated feline atria