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Role of intracellular free Ca(II) and Zn(II) in dexamethasone-induced apoptosis and dexamethasone resistance in human leukemic CEM cell lines

✍ Scribed by Dr. Foluso Adebodun; Jan F. M. Post

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
713 KB
Volume
163
Category
Article
ISSN
0021-9541

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✦ Synopsis

The levels of intracellular free Ca(ll) and Zn(ll) during dexamethasone (dex)induced apoptosis in CEM cell lines were determined by '"F nuclear magnetic resonance (NMR), using the fluorinated intracellular chelator 1,2-bis-(2-amino-5-f1uorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5-FBAPTA). The effects of these divalent metal ions on growth rate and DNA degradation were evaluated. Measurements were done on one dex-sensitive (CEM-C7) and three different dex-resistant variants (CEM-C1, CEM-4R4, and CEM-ICR27). Dex caused a continuous increase in the Ca(ll) level in dex-sensitive CEM-C7 cells, while in CEM-C1 cells dex caused an initial increase in the Ca(ll) level which in =36 h was restored to its normal value. The intracellular Ca(ll) level in CEM-4R4 cells was not significantly affected by dex, while that of CEM-ICR27 cells decreased after dex incubation. Only the dex-sensitive CEM-C7 cells showed dex-induced DNA degradation. An intracellular free Zn(ll) level of -1 n M was measured for the dexresistant CEM-C1 cells. No detectable level of intracellular Zn(ll) was found in the 0 1995 WILEY-LISS. INC. these metal ions in the mechanism of apoptosis are still not clear McConkey et al., 1989a,b;. In rat thymocytes and other types of cells, elevated levels of intracellular

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