𝔖 Bobbio Scriptorium
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ROLE OF HEPATIC FATTY ACID:COENZYME A LIGASES IN THE METABOLISM OF XENOBIOTIC CARBOXYLIC ACIDS

✍ Scribed by Kathleen M. Knights

Book ID
108937254
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
775 KB
Volume
25
Category
Article
ISSN
0305-1870

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A radiolabeled ATP assay was developed for measuring carboxylic acid:CoA ligase activity. The assay was designed to measure the formation of [gamma-33P]pyrophosphate from [gamma-33P]ATP in the course of the reaction. The assay was linear with protein concentration, and rates as low as 1 pmol/min wer