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Role of glycoproteins in the uptake of L- 14C - leucine by a synaptosomal fraction

✍ Scribed by Magdalena Rossowska

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
305 KB
Volume
5
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

The present study deals with the effect of modification of membrane glycoproteins on the accumulation of amino acids by isolated synaptosomal fractions, measured by the uptake of ^14^C‐leucine. Superficial sugar receptors were modified by concanavalin A, neuraminidase, and neuraminidase followed by concanavalin A binding. It is demonstrated that neuraminidase treatment followed by concanavalin A binding results in significant diminution of ^14^C‐leucine uptake. A similar effect was observed in experimental hypoxia followed by concanavalin A binding to isolated synaptosomal fractions.

In this case, however, (Na^+^ο£ΏK^+^) –ATPase activity remained unchanged. These results seem to indicate that ^14^Cβˆ’leucine uptake was dependent on integrity of glycoprotein structure of synaptosomal membranes.

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A study of 3H-L-fucose-containing glycop
A study of 3H-L-fucose-containing glycoproteins of the crude synaptosomal fraction, obtained from rat brain regions at various ages
✍ Leonard G. Davis; Eric G. Brunngraber; Aryeh Routtenberg πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 343 KB

## Abstract The frontal cortex showed a marked elevation of incorporation of H‐L‐fucose into glycoproteins of the crude synaptosomal fraction at 9 days of age. The effect was observed at both 8 and 24 hr after intraperitoneal administration of radioactive fucose. The elevation was absent in the 1,