Role of exogenous reduced nitrogen and sucrose in rapid high frequency somatic embryogenesis inMedicago sativa
✍ Scribed by Eltjo G. M. Meijer; Daniel C. W. Brown
- Book ID
- 104617226
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 420 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
The effect of exogenously supplied reduced nitrogen and sucrose on high-frequency somatic embryogenesis in petiole-derived tissue cultures of a diploid and a tetraploid regenerable clone of Medicago sativa ssp.falcata was investigated. There was an absolute requirement for ammonium during embryo induction and differentiation, with 5 mM being the optimum for induction and 10-20 mM the optimum for differentiation of somatic embryos. Exogenous amino acids were not essential for differentiation and often even inhibitory, except 1 or 2 g/1 casein hydrolysate or 4.4 mM glutamine with 3.1 mM proline which, under certain conditions, resulted in increases of 20-30% in the number of embryos obtained. High and low sucrose concentrations inhibited somatic embryogenesis and there was no reason to deviate from the 3% (0.088 M) sucrose level commonly used in plant tissue culture media. Selected clones from three M. sativa cultivars showed a response similar to the highly regenerable ssp.falcata clone FI.1.