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Role of DNA Sequence in the Binding Specificity of Synthetic Basic-Helix-Loop-Helix Domains

✍ Scribed by Amy C. Beltran; Philip E. Dawson; Joel M. Gottesfeld


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
299 KB
Volume
6
Category
Article
ISSN
1439-4227

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Transcription of the genomic PHO5, PHO81 and PHO84 genes of the PHO regulon requires Pho4p and Pho2p activity, whereas transcription of PHO8 is directed by Pho4p alone. Pho4p binds to two 9-bp motifs, 5'-GCACGTGGG-3' (type 1. e.g. UASp2 of PHO5 and site D of PHO84) and 5'-GCACGTTTT-3' (type 2, e.g.