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Role of divalent cations in the resumption of meiosis of rat oocytes

✍ Scribed by Tsafriri, Alexander ;Bar-Ami, Shalom

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
307 KB
Volume
205
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Resumption of meiotic maturation was induced in follicleenclosed rat‐oocytes by treatment with the divalent cationophore A23187 (10^−5^M). However, the same effect was attained by incubation in Ca^++^ ‐ deficient medium, even in the presence of EDTA or EGTA (lmM). The stability of the first polar body was increased under Ca^++^ ‐deficient conditions. Neither the ionophore nor Ca^++^ ‐deficient medium interfered with the spontaneous maturation of isolated oocytes of the rat. The experiments with cultured follicles suggest that redistribution of divalent cations may participate in the physiological control of meiosis in mammalian oocytes.

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