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Role of divalent cations in the 3',5'-exonuclease reaction of DNA polymerase I

✍ Scribed by Han, Hyunsoo; Rifkind, Joseph M.; Mildvan, Albert S.


Book ID
127389029
Publisher
American Chemical Society
Year
1991
Tongue
English
Weight
623 KB
Volume
30
Category
Article
ISSN
0006-2960

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The requirement for both DNA polymerase
✍ Sasakawa, Chihiro ;Uno, Yuko ;Yoshikawa, Masanosuke πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 615 KB

By assaying transposition of Tn5 from lambda b221 cI857 rex::Tn5 (Berg 1977) in polA-proficient and deficient cells, both the polymerase activity and 5' to 3' exonuclease activity of DNA polymerase I have been shown to be required for transposition. This requirement could not be observed in three ot