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Role of Cumulus Cells on In Vitro Maturation of Canine Oocytes

✍ Scribed by T Otoi; T Shin; DC Kraemer; ME Westhusin

Book ID
108921773
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
563 KB
Volume
42
Category
Article
ISSN
0936-6768

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## Abstract Canine ovarian oocytes were cultured in a medium consisting of TC medium 199, fetal calf serum and antibiotics. Ninety‐nine percent of the apparently healthy oocytes were in the germinal vesicle (dictyate) stage when recovered from the ovaries; 25% of them reached metaphase I or II by 7

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## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sper