Role of carbonic anhydrase in the supply of inorganic carbon to the giant clam—zooxanthellate symbiosis

✍ Scribed by D. Yellowlees; M. L. Dionisio-Sese; K. Masuda; T. Maruyama; T. Abe; B. Baillie; M. Tsuzuki; S. Miyachi

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 876 KB
• Volume: 115
• Category: Article
• ISSN: 0025-3162
• DOI: 10.1007/bf00349368

✦ Synopsis

The mechanism whereby inorganic carbon (Ci) is acquired by the symbiotic association between the giant clam (Tridacna derasa) and zooxanthellae (Symbiodinium sp.) has been investigated. Ci in the haemolymph of the clam is in equilibrium with the surrounding sea water. The photosynthesis rate exhibited by the intact clam varies as a function of the Ci concentration in the clam haemolymph. The gill tissue contains high carbonic anhydrase activity which may be important in adjusting the Ci equilibrium between haemolymph and sea water.

Zooxanthellae (Symbiodinium sp.) isolated from the clam mantle prefer C02 to HC03 as a source of inorganic carbon. The zooxanthellae have low levels of carbonic anhydrase on the external surface of the cell; however, mantle extracts display high carbonic anhydrase activity. Carbonic anhydrase is absent from the mantle of aposymbiotic clams (T. gigas), indicating that this enzyme may be essential to the symbiosis. The enzyme is probably associated with the zooxanthellae tubes in the mantle. The results indicate that carbonic anhydrase plays an important role in the supply of carbon dioxide within the clam symbiosis.