## Abstract Cellular volume and potassium contents were determined in rat astrocytes from primary culture following suspension in isoosmotic (269 mOsm) and hypoosmotic (136 mOsm) phosphateβbuffered saline (PBS) containing various potassium concentrations. Within 1 min of suspension in hypoosmotic P
Role of astrocytes in the clearance of excess extracellular potassium
β Scribed by Wolfgang Walz
- Book ID
- 117410533
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 141 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0197-0186
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The ''glial impairment hypothesis'' states that astrocytes which change from normal into the reactive type lose their ability to clear extracellular K Ψ , which in turn leads to hyperexcitability in the gliotic tissue. As this hypothesis was never proven or disproven, the question of glial efficienc
Intracellular changes of K f , Na', and Cl-were investigated by the aid of radiotracers in primary cultures of astrocytes when extracellular K f was (1) increased from 3 to 12 mM and subsequently again decreased to 3 mM; and (2) increased from 5.4 to 54 mM with subsequent decrease to 5.4 mM. In both