ROESY with Water Flip Back for High-Field NMR of Biomolecules

✍ Scribed by D.Bruce Fulton; Feng Ni

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 138 KB
• Volume: 129
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1997.1249

✦ Synopsis

We report a version of the ROESY experiment in which saturatization of interest be transverse during the mixing delay. We tion of the water magnetization is avoided without compromising report here a ROESY pulse sequence (Fig. 1A) in which the suppression of the water signal during acquisition. Field gradient water is efficiently returned to equilibrium prior to acquisition and selective RF pulses are used to maintain precise control of by use of a combination of gradient pulses, intentional radiathe water magnetization throughout the experiment and avoid tion damping, and selective RF pulses. We have avoided the signal losses due to radiation damping and molecular diffusion use of selective excitation at the solvent frequency prior to effects. The pulse sequence includes a delay for intentional radiathe mixing period, in order to preserve information at this tion damping prior to mixing period. The optimal length of this frequency along F1, and we have introduced minimal addidelay is field and sample dependent, but easily determined from tional delays to avoid sensitivity losses due to relaxation.

the apparent linewidth of the water signal. NOESY and TOCSY Because radiation damping effects are controlled, efficient variants of the same experiment are presented which make use of identical manipulations of the water magnetization. The three recovery of water z-magnetization is obtained for the entire pulse sequences constitute a suite for which little parameter adjustphase cycle and over the entire range of t 1 evolution delays.

ment is required once one of the experiments has been configured.