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Rocks are expressed in brain tumors and are required for glioma-cell migration on myelinated axons

✍ Scribed by Patrick Oellers; Uwe Schröer; Volker Senner; Werner Paulus; Solon Thanos


Book ID
102846887
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
678 KB
Volume
57
Category
Article
ISSN
0894-1491

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✦ Synopsis


Abstract

The interactions between migrating glioma cells and myelinated fiber tracts are poorly understood. We identified that C6 glioma cells can migrate along myelinated chicken retinal axons in a novel coculture, thereby expressing small GTPases of the Rho family and serine/threonine Rho‐associated kinases (ROCKs). We found that the ROCK1 isoform is also highly expressed in native human high‐grade gliomas. Glioma cells migrated faster in vitro along myelinated axons than on laminin‐1, with the former but not the latter being specifically and reversibly blocked by the ROCK inhibitor Y27632. These data suggest that the mechanisms underlying the migration of glioma cells on myelinated axons differ from those underlying the migration on extracellular matrix molecules such as laminin‐1. © 2008 Wiley‐Liss, Inc.