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Robust single-molecule approach for counting autofluorescent proteins

✍ Scribed by Cognet, Laurent; Tardin, Catherine; Négrier, Marie-Laure Martin; Breillat, Christelle; Coussen, Françoise; Choquet, Daniel; Lounis, Brahim


Book ID
121013450
Publisher
The International Society for Optical Engineering
Year
2008
Tongue
English
Weight
174 KB
Volume
13
Category
Article
ISSN
1083-3668

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✍ Laurent Cognet; Françoise Coussen; Daniel Choquet; Brahim Lounis 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 204 KB

In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The al