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Robust Growth of Escherichia coli

โœ Scribed by Ping Wang; Lydia Robert; James Pelletier; Wei Lien Dang; Francois Taddei; Andrew Wright; Suckjoon Jun


Book ID
113562878
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
799 KB
Volume
20
Category
Article
ISSN
0960-9822

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In our studies to develop a simple and reliable method for the generation of recombinant adenoviruses (Fotadar et al . 2003), we noted that the E. coli (BJ5183 and DH5alpha) survived a heat-shock of 45 degrees C for 5 minutes in 0.1 M calcium chloride. As a result, we investigated the growth of E. c