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Robust and Highly Sensitive Fluorescence Approach for Point-of-Care Virus Detection Based on Immunomagnetic Separation

✍ Scribed by Zhao, Wei; Zhang, Wan-Po; Zhang, Zhi-Ling; He, Rui-Li; Lin, Yi; Xie, Min; Wang, Han-Zhong; Pang, Dai-Wen

Book ID
120080025
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
363 KB
Volume
84
Category
Article
ISSN
0003-2700

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A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize