RNA Technologies and Their Applications

✍ Scribed by Kuniaki Saito, Keita Miyoshi, Mikiko C. Siomi (auth.), Volker A. Erdmann, Jan Barciszewski (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2010
• Tongue: English
• Leaves: 459
• Series: RNA Technologies
• Edition: 1
• Category: Library

✦ Synopsis

RNA technologies are the driving forces of modern medicine and biotechnology. They combine the fields of biochemistry, chemistry, molecular biology, cell biology, physics, nanotechnology and bioinformatics. The combination of these topics is set to revolutionize the medicine of tomorrow. After more than 15 years of extensive research in the field of RNA technologies, the first therapeutics are ready to reach the first patients. Thus we are witnessing the birth of a very exciting time in the development of molecular medicine, which will be based on the methods of RNA technologies. This volume is the first of a series. It covers various aspects of RNA interference and microRNAs, although antisense RNA applications, hammerhead ribozyme structure and function as well as non-coding RNAs are also discussed. The authors are internationally highly respected experts in the field of RNA technologies

✦ Table of Contents

Front Matter....Pages i-xvi
The Key Features of RNA Silencing....Pages 1-28
Selected Strategies for the Delivery of siRNA In Vitro and In Vivo ....Pages 29-58
RNAi Suppression and Its Application....Pages 59-92
Strategies to Prevent siRNA-Triggered Cellular Toxicity....Pages 93-106
RNAi in Malignant Brain Tumors: Relevance to Molecular and Translational Research....Pages 107-129
Silencing Huntington’s Disease Gene with RNAi....Pages 131-160
Application of Dicer-Substrate siRNA in Pain Research....Pages 161-190
RNAi Treatment of HIV-1 Infection....Pages 191-208
Application of RNA Interference to Treat Conditions Associated with Dysregulation of Transient Receptor Potential Vanilloid 1 Channel....Pages 209-226
Harnessing RNAi-Based Functional Genomics to Unravel the Molecular Complexity Underlying Skin Pigment Variation....Pages 227-253
mRNA Structure and its Effects on Posttranscriptional Gene Silencing....Pages 255-275
Antisense RNA-Mediated Regulation of the p53 Tumor Suppressor....Pages 277-284
Antisense Oligonucleotides: Insights from Preclinical Studies and Clinical Trials....Pages 285-303
What can the New Hammerhead Ribozyme Structures Teach us About Design?....Pages 305-323
microRNA Biogenesis and its Impact on RNA Interference....Pages 325-354
MicroRNAs in Epithelial Antimicrobial Immunity....Pages 355-367
Emerging Roles of Long Noncoding RNAs in Gene Expression and Intracellular Organization....Pages 369-391
Noncoding RNAs as Therapeutic Targets....Pages 393-418
Noncoding RNAs at H19 / IGF2 Locus: Role in Imprinting, Gene Expression, and Associated Pathologies....Pages 419-443
Back Matter....Pages 445-451

✦ Subjects

Molecular Medicine; Nucleic Acid Chemistry; Human Genetics; Cell Biology