RNA synthesis during spore germination in Bacillus subtilis

✍ Scribed by Sloma, A. ;Smith, I.

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 739 KB
• Volume: 175
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425526

✦ Synopsis

We have analyzed the RNA synthesized during spore germination in Bacillus subtilis. Early in germination there is little incorporation of [3H]uridine into RNA. A large increase in incorporation into RNA was found at 45 60 rain into germination which was in part due to increases in the specific activity of the UTP pool. When corrected for specific activity changes, the instantaneous rate of RNA synthesis showed a seven to tenfold increase between 30 and 45 rain of germination. Polyacrylamide gel electrophoresis studies showed that the RNA synthesized during germination appeared very similar to the RNA made during vegetative growth. DNA-RNA hybridization studies indicated that mRNA and rRNA were synthesized throughout germination. Their relative proportions remained constant and were very similar to the composition of RNA synthesized during vegetative growth.