RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells
✍ Scribed by Hiroyoshi Sawada; Masaro Tashima; Toru Nakamura; Takashi Uchiyama; Kimitaka Sagawa; Kiyoshi Takatsuki; Haruto Uchino; Yohei Ito
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- French
- Weight
- 492 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
A high molecular weight RNA‐reverse transcriptase complex in the culture media of peripheral leukocytes obtained from two Japanese patients with myeloma‐leukemia was detected by demonstration of a ^3^H‐uridine peak and a peak of DNA polymerizing activity banding at a density of 1.15–1.19 g/ml. The enzyme in the complex was able to utilize poly(rA) · d(pT)~10~ or poly (rC) · d(pG)~12–18~, but not poly (dA) · d(pT)~10~ or (dT)~12–18~, as template‐primers. The sucrose density sedimentation analysis revealed that RNA in the complex sedimented at a location of approximately 50s and 20–30s.