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RNA release from isolated brain cell nuclei: Influence of the postnatal cerebral development

✍ Scribed by Dr. Alicia E. Ramirez De Guglielmone; Beatriz H. Duvilanski

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
660 KB
Volume
3
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

A method to measure the β€œin vitro” RNA release from rat brain cell nuclei was described. Nuclear RNA was prelabelled β€œin vivo” for 30 or 120 min. In the first case the released RNA was heterogeneous and its electrophoretic mobility was similar to that of cytoplasmic messenger RNAs; nuclei prelabelled for 120 min mostly released the two major species of ribosomal RNAs. The release of mRNA form the nuclei increased during cerebral development while that of the ribosomal RNAs did not. The increased capacity of the nuclei to release β€œrapidly labeled” RNA with age neither determined an increase of the polysomal population, nor seemed to be dependent on cytoplasmic macromolecules.

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In searching for regulatory mechanisms involved in the cell-specific neuronal and glial transcription a cell-free transcriptional system has been developed using neuronal and glial rat brain chromatin and partially purified neuronal and glial nuclear rat brain RNA polymerases. Both free and chromati