RNA Processing Part B: Specific Methods

✍ Scribed by James E. Dahlberg, John N. Abelson (Eds.)

• Publisher: Academic Press
• Year: 1990
• Tongue: English
• Leaves: 689
• Series: Methods in Enzymology 181
• Edition: 1
• Category: Library

✦ Synopsis

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences

✦ Table of Contents

Content:
Contributors to volume 181
Pages ix-xii

Preface
Pages xiii-xiv
James E. Dahlberg, John N. Abelson

Volumes in series
Pages xv-xxix

Preparation and fractionation of mammalian extracts active in pre-mRNA splicing Original Research Article
Pages 3-19
Angela Krämer, Walter Keller

Small-scale preparation of extracts from radiolabeled cells efficient in pre-mRNA splicing Original Research Article
Pages 20-30
Kevin A.W. Lee, Michael R. Green

Preparation of active nuclei Original Research Article
Pages 30-36
William F. Marzluff

Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes Original Research Article
Pages 36-43
Elsebet Lund, Philip L. Paine

Transient transfection of HeLa cells for analysis of splicing reaction Original Research Article
Pages 43-48
Markus Aebi

Preparation of mammalian extracts active in polyadenylation Original Research Article
Pages 49-74
Claire L. Moore

Histone-specific RNA 3′ processing in nuclear extracts from mammalian cells Original Research Article
Pages 74-89
Claudia Stauber, Dominique Soldati, Bernhard LГјscher, Daniel SchГјmperli

Preparation and fractionation of yeast splicing extract Original Research Article
Pages 89-96
Soo-Chen Cheng, Andrew Newman, Ren-Jang Lin, Gloria D. McFarland, John N. Abelson

Affinity chromatography with biotinylated RNAs Original Research Article
Pages 97-121
S.W. Ruby, S.E. Goelz, Z. Hostomsky, J.N. Abelson

Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency Original Research Article
Pages 122-147
B.C. Rymond, C. Pikielny, B. Seraphin, P. Legrain, M. Rosbash

Analysis of pre-mRNA processing in transfected plant protoplasts Original Research Article
Pages 148-161
Gregory J. Goodall, Karin Wiebauer, Witold Filipowicz

Polyadenylate polymerases Original Research Article
Pages 161-170
Mary Edmonds

Purification and use of vaccinia virus messenger RNA capping enzyme Original Research Article
Pages 170-180
Stewart Shuman, Bernard Moss

RNA lariat debranching enzyme as tool for analyzing RNA structure Original Research Article
Pages 180-188
Barbara Ruskin, Michael R. Green

Escherichia coli ribonuclease III Original Research Article
Pages 189-202
Hugh D. Robertson

Messenger RNA turnover in cell-free extracts Original Research Article
Pages 202-209
Gary Brewer, Jeffrey Ross

Nuclear cap binding protein from HeLa cells Original Research Article
Pages 209-215
Mutsuhito Ohno, Yoshiro Shimura

Purification of small nuclear ribonucleoprotein particles active in RNA processing Original Research Article
Pages 215-232
Angela Krämer

Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs Original Research Article
Pages 232-257
Montserrat Bach, Peter Bringmann, Reinhard LГјhrmann

Isolation of small nuclear ribonucleoprotein polypeptides Original Research Article
Pages 257-263
Sallie O. Hoch

Purification and characterization of U small nuclear ribonucleoproteins in cesium chloride gradients Original Research Article
Pages 264-273
Claude Brunel, Guy Cathala

Structural analysis of U small nuclear ribonucleoproteins by in vitro assembly Original Research Article
Pages 273-284
Jörg Hamm, Iain W. Mattaj

Site-specific degradation of RNA of small nuclear ribonucleoprotein particles with complementary oligodeoxynucleotides and RNase H Original Research Article
Pages 284-292
Angela Krämer

Isolation and in vitro assembly of nuclear ribonucleoprotein particles and purification of core particle proteins Original Research Article
Pages 293-307
Stanley F. Barnett, Stephanie J. Northington, Wallace M. LeStourgeon

Physical methods for characterization of heterogeneous nuclear ribonucleoprotein complexes Original Research Article
Pages 307-317
Jerzy T. Schöneich, John O. Thomas

Immunological methods for purification and characterization of heterogeneous nuclear ribonucleoprotein particles Original Research Article
Pages 317-325
SerafГ­n PiГ±ol-Roma, Yang Do Choi, Gideon Dreyfuss

Purification and characterization of proteins of heterogeneous nuclear ribonucleoprotein complexes by affinity chromatography Original Research Article
Pages 326-331
SerafГ­n PiГ±ol-Roma, Maurice S. Swanson, Michael J. Matunis, Gideon Dreyfuss

Purification and characterization of polyadenylate-binding protein Original Research Article
Pages 332-352
Alan B. Sachs, Roger D. Kornberg

Preparation of extracts and assay of ribosomal RNA maturation in Escherichia coli Original Research Article
Pages 355-366
Anand K. Srivastava, David Schlessinger

Ribosomal RNA terminal maturase: Ribonuclease M5 from Bacillus subtilis Original Research Article
Pages 366-374
Norman R. Pace, Bernadette Pace

Yeast suppressor mutations and transfer RNA processing Original Research Article
Pages 377-394
Mark Nichols, Ian Willis, Dieter Söll

Genetics of transfer RNA processing in Escherichia coli Original Research Article
Pages 395-400
Yoshiro Shimura, Hideaki Shiraishi

Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs Original Research Article
Pages 400-421
Anita K. Hopper

Ribonucleases active at 3′ terminus of transfer RNA Original Research Article
Pages 421-433
Murray P. Deutscher

Transfer RNA nucleotidyltransferase Original Research Article
Pages 434-439
Murray P. Deutscher

Yeast extracts for transfer RNA gene transcription and processing Original Research Article
Pages 439-450
Claire F. Evans, David R. Engelke

Enzymatic addition of guanylate to histidine transfer RNA Original Research Article
Pages 451-462
Jacinta B. Williams, Lynn Cooley, Dieter Söll

Purification of yeast transfer RNA ligase Original Research Article
Pages 463-471
Qi Xu, Eric M. Phizicky, Chris L. Greer, John N. Abelson

Highly purified transfer RNA splicing endonuclease from Saccharomyces cerevisiae Original Research Article
Pages 471-480
Phillip R. Green, John N. Abelson

Purification and characterization of wheat germ RNA ligase and associated activities Original Research Article
Pages 480-499
Leslie Pick, Henry M. Furneaux, Jerard Hurwitz

RNA 3′-terminal phosphate cyclase from HeLa cells Original Research Article
Pages 499-510
Witold Filipowicz, Oscar Vicente

Transfer RNA splicing endonuclease from Xenopus laevis Original Research Article
Pages 510-517
D. Gandini-Attardi, I.M. Baldi, M. Mattoccia, G.P. Tocchini-Valentini

Genetic and molecular analysis of RNA splicing in Escherichia coli Original Research Article
Pages 521-539
Marlene Belfort, Karen Ehrenman, P.Scott Chandry

Genetic analysis of RNA splicing in yeast mitochondria Original Research Article
Pages 539-558
Philip S. Perlman

Self-splicing and enzymatic cleavage of RNA by a group I intervening sequence Original Research Article
Pages 558-569
John A. Latham, Arthur J. Zaug, Thomas R. Cech

Preparation and characterization of RNase P from Escherichia coli Original Research Article
Pages 569-582
Madeline F. Baer, John G. Arnez, Cecilia Guerrier-Takada, Agustin Vioque, Sidney Altman

Characterization of self-cleavage of viroid and virusoid RNAs Original Research Article
Pages 583-607
Anthony C. Forster, Christopher Davies, Cheryl J. Hutchins, Robert H. Symons

Author index
Pages 609-631

Subject index
Pages 633-651