RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria

Isolation and phenotypic analysis of con

Isolation and phenotypic analysis of conditional-lethal, linker-insertion mutations in the gene encoding the largest subunit of RNA polymerase II in Saccharomyces cerevisme

✍ Archambault1, Jacques ;Drebot, MichaelA. ;Stone, JamesC. ;Friesen, JamesD. 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 947 KB

Linker-insertion mutagenesis was used to isolate mutations in the Saccharomyces cerevisiae gene encoding the largest subunit of RNA polymerase II (RPO21, also called RPB1). The mutant rpo21 alleles carried on a plamid were introduced into a haploid yeast strain that conditionally expresses RPO21 fro