Content:
Front Matter, Page iii
Copyright, Page iv
Dedication, Page v
Preface, Pages xiii-xiv
CHAPTER 1 - RNA and the Cellular Biochemistry Revisited, Pages 1-10
CHAPTER 2 - Transcription and the Organization of Eukaryotic Gene Sequences, Pages 11-19
CHAPTER 3 - Messenger RNA, Pages 20-31
CHAPTER 4 - Resilient Ribonucleases, Pages 32-45
CHAPTER 5 - RNA Isolation Strategies, Pages 46-92
CHAPTER 6 - Isolation of Polyadenylated RNA, Pages 93-111
CHAPTER 7 - Electrophoresis: Principles and Parameters, Pages 112-124
CHAPTER 8 - Electrophoresis of RNA, Pages 125-157
CHAPTER 9 - The Northern Blot, Pages 158-173
CHAPTER 10 - Nucleic Acid Probe Technology, Pages 174-190
CHAPTER 11 - Practical Nucleic Acid Hybridization, Pages 191-203
CHAPTER 12 - Principles of Detection, Pages 204-219
CHAPTER 13 - Quantitation of Specific Messenger RNAs by the S1 Nuclease Assay, Pages 220-234
CHAPTER 14 - Analysis of Nuclear RNA, Pages 235-263
CHAPTER 15 - An RNA Paradigm, Pages 264-270
Epilogue - A Few Pearls of Wisdom, Pages 271-274
APPENDIX A - Deionization of Glyoxal, Pages 275-276
APPENDIX B - Phenol Preparation, Pages 277-280
APPENDIX C - Deoxyribonuclease I Incubation to Remove DNA from an RNA Sample, Pages 281-282
APPENDIX D - RNase Incubation to Remove RNA from a DNA Sample, Pages 283-284
APPENDIX E - Silanizing Centrifuge Tubes and Glassware, Pages 285-286
APPENDIX F - Treatment of Ethidium Bromide Solutions, Pages 287-288
APPENDIX G - Trypsinization Protocol, Pages 289-290
APPENDIX H - Isolation of High Molecular Weight DNA by the Salting-Out Procedure, Pages 291-293
APPENDIX I - Agarose Gel Electrophoresis with Methylmercuric Hydroxide, Pages 294-296
APPENDIX J - Polyacrylamide Gel Electrophoresis of RNA, Pages 297-299
APPENDIX K - Major Suppliers of Equipment and Reagents, Pages 300-303
Glossary, Pages 304-310
Index, Pages 311-317