RNA-dependent RNA polymerase encoded by hepatitis C virus: biomedical applications
✍ Scribed by V. J.-P. Lévêque; Q. M. Wang
- Book ID
- 105757504
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 175 KB
- Volume
- 59
- Category
- Article
- ISSN
- 1420-682X
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## Abstract Since the amount of hepatitis C virus (HCV) RNA might be correlated with the degree of severity of hepatitis and response to treatment, quantitation of HCV RNA in serum was established using competitive polymerase chain reaction (PCR). Known amounts of a plasmid containing HCV‐cDNA were
Although detection of hepatitis C virus RNA with polymerase chain reaction has become the standard for diagnosis, extensive application has been thwarted by polymerase chain reaction's labor intensiveness, risk of false-positive results through contamination and time required for individual assays.