RNA-dependent DNA polymerase associated with a simian lymphoid cell line derived from a Herpesvirus saimiri-induced lymphoma

✍ Scribed by Stringner S. Yang; D. Ablashi; G. Armstrong; R. C. Ting

• Book ID: 102866977
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 653 KB
• Volume: 13
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910130110

✦ Synopsis

Abstract

This communication describes the spontaneous appearance of a membrane‐bound RNA‐dependent DNA polymerase in a simian lymphoma cell line that was established from a lymphoma tumor mass previously induced by Herpesvirus saimiri in an owl monkey. This membrane‐bound RNA‐dependent DNA polymerase fraction possesses many biochemical properties analogous to those of known primate and murine RNA tumor viruses. Characteristics of this RNA‐dependent DNA polymerase are clearly distinct from those of the DNA polymerase of Herpesvirus saimiri. This particular fraction incorporates tritiated ribonucleosides into RNA in an in vitro system, and it sediments at 1.15–1.16 g/cm^3^. It is not induced to greater production by halogenated deoxyuridines. The current observations were made at a time when the host cells were no longer producing Herpesvirus saimiri.