Ring-paper chromatographic technique for the separation of tobacco alkaloids
β Scribed by Swarup Narain Tewari
- Publisher
- Springer
- Year
- 1954
- Tongue
- English
- Weight
- 146 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0028-1042
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Ruorescence detection has been used for the detection of peptides that contain tyrosine or tryptophan. The strong native fluorescence of these groups makes this method several times more sensitive than detection at 254 nm. Furthermore, when combined in series with a lower wavelength absorbance detec
The 0.05 M phthslatc buffer was preparecl by mixing 50 ml of 0.1 M potassium acicl phthalato and 45.45 ml of 0.1 N NsOH and diluting to IOO ml with CO,-free waferG. l \* All the DNP-amino acicls used in this study were preparccl by the method of SANGEIZ~ or that of LEVY AND CHTJNG'.