Wild-type tobacco (Nicotiana tabacum L.) plants and transgenic tobacco transformed with antisense rbcS to decrease expression of ribulose-l,5-bisphosphate carboxylase-oxygenase (Rubisco; EC 4.1.1.39) were grown at 300mol-m-2.s -1 irradiance and 20~ at either 0.1, 0.7 or 5 mM NH4NO 3. In high nitroge
Ribulose-1,5-bisphosphate carboxylase/oxygenase expression in melon plants infected withColletotrichum lagenarium
✍ Scribed by Benoit Ranty; Dominique Roby; Gérard Cavalié; Marie-Thérèse Esquerré-Tugayé
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 917 KB
- Volume
- 170
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Ribulose-l,5-bisphosphate carboxylase/ oxygenase (RuBPCase) was studied in melon leaves infected by Colletotrichum lagenarium, a fungal pathogen of melons. Electrophoretic analysis of melon leaf proteins indicated a strong effect of infection on RuBPCase, the subunits of which gradually disappeared during the different stages of infection. Enzyme activity also declined 4 d after inoculation and its content, measured by immunoelectrophoresis, decreased to a similar extent. Synthesis of the large and small subunits of RuBPCase was followed by in-vivo pulse-labeling experiments. A drastic decrease in the rate of RuBPCasesubunit synthesis occurred 3 d after inoculation and preceded the appearance of disease symptoms. There was an apparent coordination of the synthesis of the two subunits under these conditions.
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