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Ribulose-1,5-bisphosphate carboxylase/oxygenase expression in melon plants infected withColletotrichum lagenarium

✍ Scribed by Benoit Ranty; Dominique Roby; Gérard Cavalié; Marie-Thérèse Esquerré-Tugayé


Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
917 KB
Volume
170
Category
Article
ISSN
0032-0935

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✦ Synopsis


Ribulose-l,5-bisphosphate carboxylase/ oxygenase (RuBPCase) was studied in melon leaves infected by Colletotrichum lagenarium, a fungal pathogen of melons. Electrophoretic analysis of melon leaf proteins indicated a strong effect of infection on RuBPCase, the subunits of which gradually disappeared during the different stages of infection. Enzyme activity also declined 4 d after inoculation and its content, measured by immunoelectrophoresis, decreased to a similar extent. Synthesis of the large and small subunits of RuBPCase was followed by in-vivo pulse-labeling experiments. A drastic decrease in the rate of RuBPCasesubunit synthesis occurred 3 d after inoculation and preceded the appearance of disease symptoms. There was an apparent coordination of the synthesis of the two subunits under these conditions.


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