Ribosome slowed by mutation to streptomycin resistance
โ Scribed by GALAS, DAVID J.; BRANSCOMB, ELBERT W.
- Book ID
- 109702057
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 466 KB
- Volume
- 262
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/262617b0
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๐ SIMILAR VOLUMES
Peptidyl-tRNA may dissociate preferentially from ribosomes during protein synthesis when it is inappropriate to, does not correctly complement, the messenger RNA. To test this idea, growing cultures of Escherichia coli were treated with streptomycin to increase the frequency of errors during protein
Streptomycin-resistant and dependent mutants were isolated from Bacillus subtilis ATCC 6633. Ribosomal proteins were analysed from six such mutants with chromatography on carboxymethyl cellulose or phosphocellulose columns. An altered specific 30s ribosomal protein, 30B, could be detected in all of