Ribosome display selection of a metal-binding motif from an artificial peptide library

✍ Scribed by Akira Wada; Shinya Y. Sawata; Yoshihiro Ito

• Book ID: 101721059
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 169 KB
• Volume: 101
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21975

✦ Synopsis

Abstract

A new ribosome display system was applied for the in vitro selection of a metal‐binding motif from an artificial peptide library. The display system consisted of an mRNA‐associating protein, a ribosome, and mRNA. The protein part of this display system was designed to provide a random peptide library and to stabilize the ribosome display. The random peptide library was newly designed to isolate stable metal‐binding motifs. We employed the system for in vitro selection and found several new proteins and peptides that bind Co(II)‐immobilized resin and Co(II)‐complex, respectively. This newly developed system can be conveniently applied to the in vitro selection of peptide aptamers. Biotechnol. Bioeng. © 2008 Wiley Periodicals, Inc.