## Abstract We have measured the turnover rate of ribosomal RNA in exponentially growing __Tetrahymena thermophila__ cells, cells entering the plateau phase of growth, and nutrient‐deprived (starved) cells. Ribosomal RNA is stable in cells in early log phase growth but it begins to turnover as the
Ribosome biosynthesis in Tetrahymena thermophila. IV. Regulation of ribosomal RNA synthesis in growing and growth arrested cells
✍ Scribed by Claudia A. Sutton; Paul Sylvan; Richard L. Hallberg
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 891 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Three parameters involved in the production of new ribosomal RNA (rRNA) were measured in Tetrahymena thermophilia: (i) the rate of synthesis of the rRNA precursor, (ii) the rate of processing of the RNA precursor and rRNA intermediates and (iii) the efficiency of utilization of the rRNA precursor in producing mature ribosomal RNA. These parameters were measured in cells in exponential growth and in cells starved in a dilute salt solution. Growing cells synthesize rRNA 20 times faster and process rRNA precursors and intermediates 10 to 15 times more rapidly than do starved cells. Both utilize their rRNA precursors with an efficiency of one in converting them to mature rRNA.
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