Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology
β Scribed by Rudolf Amann; Wolfgang Ludwig
- Book ID
- 114172243
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 813 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1574-6976
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We have constructed light-up probes for nucleic acid detection. The light-up probe is a peptide nucleic acid (PNA) oligonucleotide to which the asymmetric cyanine dye thiazole orange (TO) is tethered. It combines the excellent hybridization properties of PNA and the large fluorescence enhancement of
## Abstract Laserβexcited Raman spectra of tRNA^Phe^ from yeast and of fractionated 16S and 23S rRNA from __E. coli__ are reported for samples in aqueous solution and in the solid state. The Raman scattering spectrum of each RNA is not significantly altered by the change from an aqueous to a solid