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Ribosomal rna in avian leukosis virus particles

✍ Scribed by Tadashi Obara; Dani P. Bolognesi; Heinz Bauer

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
907 KB
Volume
7
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The RNAs of avian viruses originating from the plasma of leukemic chickens (AMV) or from tissue culture (MAV‐B) consisted of five distinct components with sedimentation constants of 62 s, 27 s, 17 s, 9 s, and 4–5 s. Whereas the 27 s and 17 s minor RNA components could be consistently recovered from large quantities of AMV, fractionation procedures as well as labelling conditions were found to be critical for their detection when the virus originated from tissue culture fluids. Sedimentation studies, base ratio analysis, and hybridization experiments all indicated that the 27 s and 17 s components probably represent ribosomal RNA, and the significance of the presence of cellular RNA in the virus particles is discussed.

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