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Ribosomal and non-ribosomal protective components isolated fromListeria monocytogenes

โœ Scribed by A. C. J. M. Antonissen; P. J. M. R. Lemmens; R. Kolfschoten-Gonggrijp; J. F. Bosch; C. P. A. Boven


Book ID
104757748
Publisher
Springer Netherlands
Year
1983
Tongue
English
Weight
58 KB
Volume
49
Category
Article
ISSN
0003-6072

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A general procedure for purifying and efficiently separating four types of DNA from filamentous fungi has been developed. The protocol involves (i) disruption of mycelial cells by blending in liquid nitrogen followed by suspension of cell contents in buffer containing high concentrations of protease