Ribosomal acidic proteins of eucaryotic cells

✍ Scribed by Nadezda P. Yurina; Nadezda A. Byzova; Margarita S. Odintsova

• Book ID: 104671627
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 559 KB
• Volume: 50
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00225277

✦ Synopsis

By the method of ethanol-salt extraction with ion-exchange chromatography on CM-cellulose an acidic protein of pea 80S ribosomes was isolated. This protein located in the large subunit, had a molecular weight of 14 000 and an IEP of 4.7. The protein is partially phosphorylated, alanine-rich and has methionine at the N-terminal position. Based on these characteristics and on the comparative study of tryptic hydrolyzates of the plant protein and E. coli L7/L12, the protein so obtained is found to be homologous to the L7/L12 of the procaryotic ribosomes.