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Ribonucleinase and non-specific phosphodiesterase in rat and rabbit blood and tissues

โœ Scribed by Charles A. Zittle; Elizabeth H. Reading

Publisher
Elsevier Science
Year
1946
Tongue
English
Weight
262 KB
Volume
242
Category
Article
ISSN
0016-0032

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โœฆ Synopsis

Ribonucleinase and Non-Specific Phosphodiesterase in Rat and

Rabbit Blood and Tissues.--CHARLES A. ZITTLE AND ELIZABETH H. READING. A manometric method for estimating crystalline ribonucleinase was devised by Bain and Rusch (I), and applied to biological material. This method was subsequently used by others (2) who in later studies (3,4) showed that a purified non-specific phosphodiesterase from calf intestinal mucosa could be estimated by the same procedure. This finding raised the question as to which enzyme was being measured in biological material. The two enzymes were distinguished (3) by the large amount of substrate required'for the full activity of the former and the small amount required for the latter. This property has been used to differentiate these enzymes in the blood and tissues of rat and rabbit.

EXPERIMENTAL.

๐Ÿ“œ SIMILAR VOLUMES

Abstract of Ribonucleinase. I. Manometri
Abstract of Ribonucleinase. I. Manometric Determination of Ribonucleinase in Blood and Tissues of the Rat and the Rabbit
๐Ÿ“‚ Article ๐Ÿ“… 1946 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 46 KB

Most of the enzyme was found to be in the blood cells. Assays were also performed on the bone marrow, spleen, and pancreas of the rat and the rabbit. Solubility studies yielded confirming eyidence that the nucleic acid is broken down by the blood.