The maintenance and genetic stability of the vector plasmids pBR322 and pBR325 in two genetically different Escherichia coli hosts were studied during chemostat cultivation with glucose and ammonium chloride limitation and at two different dilution rates. The plasmid pBR322 was stably maintained und
Ribitol dehydrogenase overproduction and maintenance of plasmids pBR322 and pACYC184 in chemostat cultures of Escherichia coli
✍ Scribed by P. Kyslík; L. Sobotková; B. Večerek; M. Dobišová; T. Chrudimský
- Publisher
- Springer Netherlands
- Year
- 1989
- Tongue
- English
- Weight
- 246 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0141-5492
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## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP