rhG-CSF affects genes involved in mitogen signalling and early gene expression in the ovarian cancer cell line HEY
✍ Scribed by Thomas Brandstetter; Elena Ninci; Ulrich Falken; Edgar Wagner; Ralf Hess; Thomas Bauknecht
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- French
- Weight
- 435 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
The ovarian adenocarcinoma cell line HEY was used as an in vitro model to study the influence of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on epithelial tumours such as ovarian cancer. Serum-starved cells were treated with rhG-CSF in a time-and dose-dependent manner. Cell proliferation, measured as cell division and DNA synthesis, was stimulated about 40% by rhG-CSF. After harvesting, cells were examined for the presence of G-CSF receptor (FACS analysis and RT-PCR), as well as for expression of genes involved in mitogen signalling (ERKs, JNKs) and early gene expression (c-jun). rhG-CSF affected mitogenactivated pathways and was receptor-mediated if the G-CSF receptor was present. After rhG-CSF induction, Janus Nterminal kinases (JNK 1 and 2) were simultaneously increased in the cytosol, up to 30-fold as measured by Western blotting), whereas ERK 1 and 2 accumulated maximally by 2.5-fold 1 hr after rhG-CSF induction. c-Jun was up-regulated strongly by this cytokine at the translational level. Our data suggest that rhG-CSF affects genes involved in mitogen signalling and early gene expression in solid tumours. We also noted the presence of G-CSF receptor on ovarian cancer cell lines.