Rheumatoid factors from patients with rheumatoid arthritis react with tryptophan 60 and 95, lysine 58, and arginine 97, on human β2-microglobulin
✍ Scribed by Ralph C. Williams Jr; Christine C. Malone; John B. Harley
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 1022 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
To define precise epitopes on human P2-microglobulin (P2m) reacting with polyclonal IgM rheumatoid factors (RF) from patients with rheumatoid arthritis (RA).
Methods. Ten polyclonal RF were tested for their human P2m epitop+binding specificities using the entire 99-amino acid sequence synthesized as overlapping 7-mers in an enzyme-linked immunosorbent assay. Glycine substitution for each residue within RF-reacting linear regions was employed to define major reactive sites.
Results and Conclusion.
Major Pzm residues contributing to RF reactivity were tryptophans at positions 60 and 95, lysine at 58, and arginine at position 97.
Human IgM rheumatoid factors (RFs) probably represent the first autoantibody ever recognized (1,2), and since their initial description more than 50 years From the